Toll-Like Receptors by Claire E. McCoy

Author:Claire E. McCoy
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

10.qPCR primers for lineage-specific cytokines and transcription factors have been described previously [15, 19]. If performing relative mRNA analysis, be sure to include primers for housekeeping reference genes such as β-actin or GAPDH.

11.In addition to CD4+ T cells, we have also adopted these methods for the study of TLRs on γδ T cells and CD8+ T cells [15, 19].

12.Proliferation assays using 3H-thymidine incorporation are not as common due to the dangers of handling radioactive materials and the cost of the necessary equipment. If your facility is not equipped for this type of experiment, many commercially available kits exist for assaying cell proliferation, including the use of CFDA as outlined in this protocol.

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